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KMID : 1149220170250020179
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Korean Journal of Oriental Medical Prescription
2017 Volume.25 No. 2 p.179 ~ p.191
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Protective Effects of Ethanol Extract Mixtures of Sophora flavescens, Glycyrrhiza uralensis and Dictamnus dasycarpus against Oxidative Stress-induced Damage in C2C12 Murine Myoblasts
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Choi Eun-Ok
Hwang Young-Hoon
Kim Min-Young
Son Da-Hee
Jeong Jin-Woo
Park Cheol
Hong Su-Hyun
Kim Min-Ju
Lee Ji-Young
Choi Yung-Hyun
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Abstract
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Objectives : Increased oxidative stress by reactive oxygen species (ROS) has been suggested as a major cause of muscle fatigue. Although several studies have demonstrated the various biological properties of Sophora flavescens Aiton, Glycyrrhiza uralensis Fischer and Dictamnus dasycarpus Turcz, but the antioxidative potentials have not been clearly demonstrated. The present study was designed to investigate the protective effects of their water and ethanol extract mixtures (medicinal herbal mixtures, MHMIXs) on hydrogen peroxide (H2O2)-induced cell damage and apoptosis in C2C12 myoblasts.
Methods : Cytotoxicity was assessed by an MTT assay. Quantitative evaluation of apoptosis induction and ROS production was evaluated by flow cytometry analysis. Expression levels of apoptosis regulatory and DNA-damage proteins were detected by Western blotting.
Result : The inhibition of H2O2-induced cell proliferation was effectively blocked in extracts of 3: 1: 1 (EMHMIXs-1) or 2: 2: 1 (EMHMIXs-2) of S. flavescens, G. uralensis and D. dasycarpus Turcz, ethanol extracts from various complex extracts in C2C12 myoblasts. EMHMIXs-1 and EMHMIXs-2 also effectively attenuated H2O2-induced C2C12 cell apoptosis, which was associated with the restoration of the upregulation of Bad and death receptor 4, and downregulation of XIAP and cIAP-1 induced by H2O2. In addition, these herbal mixtures significantly blocked the H2O2-induced ROS generation and phosphorylation of p-γH2A.X, which suggests that they can prevent H2O2-induced cellular DNA damage.
Conclusions : The results suggest that EMHMIXs-1 and EMHMIXs-2 could block the DAN damage and apoptosis of C2C12 myoblasts by oxidative stress through blocking ROS generation.
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KEYWORD
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C2C12 myoblasts , medicinal herbal mixtures , oxidative stress , DNA damage , apoptosis
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